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pdh-e1 ser293 phosphorylation  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pdh-e1 ser293 phosphorylation
    Pdh E1 Ser293 Phosphorylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdh-e1+ser293+phosphorylation/pdh+antibody/pm28028037-89-18-8
    Average 90 stars, based on 1 article reviews
    pdh-e1 ser293 phosphorylation - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Muscle interleukin-6 and fasting-induced PDH regulation in mouse skeletal muscle.
    Article Snippet: 4511, 2535, 9252, 9251, D22A3, and 9441, respectively; Cell Signaling Technologies, Danvers, MA); acetyl-CoA carboxylase (ACC)Ser212 phosphorylation and PDH-E1 Ser232 phosphorylation (07–303 and no. AP1063, respectively; EMD Millipore, Bedford, MA); PDK1 (ab90444, Abcam, Cambridge, UK); PDK2 protein (ST1643, Calbiochem); and PDP1 protein (Sigma-Aldrich, St. Louis, MO).



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    <t>A.</t> <t>P-PDH/total</t> PDH ratio from WT and MCU OE retinas, normalized to WT. MCU OE retinas have a 1.12 ± 0.02-fold higher P-PDH/total PDH ratio than WT. n=6 WT and 6 MCU OE retinas. *p<0.05 using Welch’s t-test. B. Diagram showing how labelled carbons from U- 13 C-glucose are incorporated through glycolysis and the first round of the TCA cycle. Shaded = labeled carbon, empty = unlabeled carbon. C. Levels of isotopomers in WT and MCU OE retinas. ‘m’ signifies the number of 13 C-labeled carbons in each metabolite. ‘m2’ TCA cycle metabolites are made from one round of the TCA cycle. Data points represent averages from n=3 retinas from 3 different fish. *p<0.05 using Welch’s t-test.
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    Cell Signaling Technology Inc pdh-e1 ser293 phosphorylation
    <t>A.</t> <t>P-PDH/total</t> PDH ratio from WT and MCU OE retinas, normalized to WT. MCU OE retinas have a 1.12 ± 0.02-fold higher P-PDH/total PDH ratio than WT. n=6 WT and 6 MCU OE retinas. *p<0.05 using Welch’s t-test. B. Diagram showing how labelled carbons from U- 13 C-glucose are incorporated through glycolysis and the first round of the TCA cycle. Shaded = labeled carbon, empty = unlabeled carbon. C. Levels of isotopomers in WT and MCU OE retinas. ‘m’ signifies the number of 13 C-labeled carbons in each metabolite. ‘m2’ TCA cycle metabolites are made from one round of the TCA cycle. Data points represent averages from n=3 retinas from 3 different fish. *p<0.05 using Welch’s t-test.
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    A. P-PDH/total PDH ratio from WT and MCU OE retinas, normalized to WT. MCU OE retinas have a 1.12 ± 0.02-fold higher P-PDH/total PDH ratio than WT. n=6 WT and 6 MCU OE retinas. *p<0.05 using Welch’s t-test. B. Diagram showing how labelled carbons from U- 13 C-glucose are incorporated through glycolysis and the first round of the TCA cycle. Shaded = labeled carbon, empty = unlabeled carbon. C. Levels of isotopomers in WT and MCU OE retinas. ‘m’ signifies the number of 13 C-labeled carbons in each metabolite. ‘m2’ TCA cycle metabolites are made from one round of the TCA cycle. Data points represent averages from n=3 retinas from 3 different fish. *p<0.05 using Welch’s t-test.

    Journal: bioRxiv

    Article Title: Mitochondrial Ca 2+ influences photoresponse recovery, metabolism, and mitochondrial localization in cone photoreceptors

    doi: 10.1101/510792

    Figure Lengend Snippet: A. P-PDH/total PDH ratio from WT and MCU OE retinas, normalized to WT. MCU OE retinas have a 1.12 ± 0.02-fold higher P-PDH/total PDH ratio than WT. n=6 WT and 6 MCU OE retinas. *p<0.05 using Welch’s t-test. B. Diagram showing how labelled carbons from U- 13 C-glucose are incorporated through glycolysis and the first round of the TCA cycle. Shaded = labeled carbon, empty = unlabeled carbon. C. Levels of isotopomers in WT and MCU OE retinas. ‘m’ signifies the number of 13 C-labeled carbons in each metabolite. ‘m2’ TCA cycle metabolites are made from one round of the TCA cycle. Data points represent averages from n=3 retinas from 3 different fish. *p<0.05 using Welch’s t-test.

    Article Snippet: Phosphorylated Pyruvate Dehydrogenase E1 subunit Ser293, P-PDH (EMD Millipore, ABS204, RRID:AB_11205754) Used in immunoblotting at 1:2000 dilution.

    Techniques: Labeling

    A. Immunoblot showing P-PDH and total PDH expression in WT and MCU OE retinas. n=6 WT and 6 MCU OE retinas from 3 different fish. B. Immunoblot of mtCO1, SDH, and Pyruvate Kinase (PK) in WT and MCU OE retinas. We observed that expression of every protein we probed for (PDH, SDH, mtCO1 and pyruvate kinase) was slightly lower in MCU OE retinas, even when the same amount of protein lysate was loaded. We hypothesize that this is due to the fact that in MCU OE retinas, MCU and RFP comprise a much larger fraction of the total protein, so other proteins appear less abundant when normalizing to total protein. n=4 WT and 4 MCU OE retinas from 4 different fish. C. Total metabolite levels in freshly dissected WT zebrafish retinas and relative levels of these metabolites in MCU OE retinas. (BHB: β-hydroxybutyrate, 3PG: 3-phosphoglycerate, PEP: phosphoenolpyruvate). D. Glycolytic intermediates from WT and MCU OE retinas supplied with 13 C-glucose. We observed no trends of altered glycolytic flux over time between WT and MCU OE retinas. * p < 0.05 using Welch’s t-test.

    Journal: bioRxiv

    Article Title: Mitochondrial Ca 2+ influences photoresponse recovery, metabolism, and mitochondrial localization in cone photoreceptors

    doi: 10.1101/510792

    Figure Lengend Snippet: A. Immunoblot showing P-PDH and total PDH expression in WT and MCU OE retinas. n=6 WT and 6 MCU OE retinas from 3 different fish. B. Immunoblot of mtCO1, SDH, and Pyruvate Kinase (PK) in WT and MCU OE retinas. We observed that expression of every protein we probed for (PDH, SDH, mtCO1 and pyruvate kinase) was slightly lower in MCU OE retinas, even when the same amount of protein lysate was loaded. We hypothesize that this is due to the fact that in MCU OE retinas, MCU and RFP comprise a much larger fraction of the total protein, so other proteins appear less abundant when normalizing to total protein. n=4 WT and 4 MCU OE retinas from 4 different fish. C. Total metabolite levels in freshly dissected WT zebrafish retinas and relative levels of these metabolites in MCU OE retinas. (BHB: β-hydroxybutyrate, 3PG: 3-phosphoglycerate, PEP: phosphoenolpyruvate). D. Glycolytic intermediates from WT and MCU OE retinas supplied with 13 C-glucose. We observed no trends of altered glycolytic flux over time between WT and MCU OE retinas. * p < 0.05 using Welch’s t-test.

    Article Snippet: Phosphorylated Pyruvate Dehydrogenase E1 subunit Ser293, P-PDH (EMD Millipore, ABS204, RRID:AB_11205754) Used in immunoblotting at 1:2000 dilution.

    Techniques: Western Blot, Expressing